CD Glucose-6-Phosphate Dehydrogenase Kit

Catalog Number: AKBA231

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$1317

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Specifications

Product Information
Product Name	CD Glucose-6-Phosphate Dehydrogenase Kit
Applications	For quantitative colorimetric determination of glucose-6-phosphate concentration and evaluation of drug effects on its metabolism.
Features	Fast and sensitive. Linear detection range (20 µL sample): 0.2 to 100 U/L for 15 min reaction. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD565nm
Sample Type	Plasma, serum, tissue and culture media, etc
Species	All
Assay Time	20 min
Limit of Detection	0.2 U/L
Expiration Date	6 months
Description	GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. BioAssay Systems’ non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in an NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
Shipping Conditions	Shipping: RT
Storage	Storage: -20°C upon receipt

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