CD Glucose Oxidase Assay Kit

Catalog Number: AKBA195

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$1197

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Specifications

Product Information
Product Name	CD Glucose Oxidase Assay Kit
Applications	For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism.
Features	Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate for 20-minute incubation at 25°C: 0.02 to 10 U/L glucose oxidase for colorimetric assays and 0.002 to 1.5 U/L for fluorimetric assays. Simple and high-throughput. The procedure involves the addition of a single working reagent and incubation for 20 min at room temperature. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD570nm, FL530/585nm
Sample Type	Cell/tissue lysate, cell culture media, etc
Species	All
Assay Time	20 min
Limit of Detection	0.02 (OD), 0.002U/L (FL)
Expiration Date	6 months
Description	Glucose oxidase catalyzes the oxidation of glucose from D-glucose to D-glucono-δ-lactone. Physiologically, it aids in the breakdown of glucose into smaller metabolites. It is widely used in electrochemical glucose sensors designed for diabetes patients. Simple, direct, and high-throughput assays for measuring glucose oxidase activity find wide applications in research and drug discovery. CD glucose oxidase assay kit uses a single Working Reagent that combines the glucose oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to glucose oxidase activity in the sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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