CD Glucose Uptake Assay Kit

Catalog Number: AKBA153

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$1338

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Specifications

Product Information
Product Name	CD Glucose Uptake Assay Kit
Applications	For quantitative determination of glucose uptake in whole cells and evaluation of effects of ligands or drugs on glucose transport.
Features	Safe. No radioactive material is used. Sensitive and Accurate. The detection limit of 0.1 µM and linearity up to 5 µM 2-DG6P. Simple and Convenient. Can be automated as a medium throughput assay for glucose transport in cells.
Detection Method	FL530/585nm
Sample Type	Cell culture
Species	Mammalian
Assay Time	Approximately 2 hrs
Limit of Detection	0.1 µM
Expiration Date	6 months
Description	Glucose Uptake has a variety of methods and transporters and depends upon the metabolic demand of the cell type and the availability of glucose. There are over ten different facilitated diffusion glucose transporters that transport glucose down its concentration gradient without ATP hydrolysis. In the kidneys, secondary active transport is used to uptake Glucose against its concentration gradient to ensure that very little glucose is excreted in the urine.CD fluorescent cell-based glucose uptake assay uses 2-deoxyglucose (2-DG), a widely used glucose analog because it can be taken up by glucose transporters and metabolized by endogenous hexokinase into 2-deoxyglucose 6-phosphate (2-DG6P). 2-DG6P accumulates intracellularly because it is not a suitable substrate for phosphoglucose isomerase, the next step in glycolysis. The cells are lysed, and excess NADP and glucose 6-phosphate dehydrogenase (G6PDH) are added to metabolize 2-DG6P and generate a molar equivalent amount of NADPH. The NADPH is then measured using a G6PDH recycling reaction to amplify the signal and generate a fluorescent signal measurable at λex/em = 530/585 nm proportional to the concentration of 2-DG6
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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