CD α-Glucosidase Assay Kit

Catalog Number: AKBA054

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$1467

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Specifications

Product Information
Product Name	CD α-Glucosidase Assay Kit
Applications	For quantitative determination of α-glucosidase activity and evaluation of drug effects on its metabolism.
Features	High sensitivity and wide linear range. Use a 20 µL sample. The detection limit is 2 U/L, linear up to 250 U/L. Homogeneous and simple procedure. A simple “mix-and-measure” procedure allows reliable quantitation of α-glucosidase activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.
Detection Method	OD405nm
Sample Type	Biological
Species	All
Assay Time	20 min
Limit of Detection	2 U/L
Expiration Date	6 months
Description	α-GLUCOSIDASE hydrolyzes the terminal, non-reducing 1,4-linked α-D-glucose residues with release of α-D-glucose. α-Glucosidase is needed by all animals to hydrolyze maltose to glucose for use as a food. Aberrant activities have been implicated in diseases such as diabetes and Pompe disease. Simple, direct, and automation-ready procedures for measuring α-glucosidase activity are becoming popular in Research and Drug Discovery. BioAssay Systems QuantiChrom™ α-Glucosidase Assay Kit is designed to measure α-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-α-D-glucopyranoside that is hydrolyzed specifically by α-glucosidase into a yellow-colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
Shipping Conditions	Shipping: RT
Storage	Storage: -20°C upon receipt

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