Product Information | |
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Product Name | CD α-Glucosidase Assay Kit |
Applications | For quantitative determination of α-glucosidase activity and evaluation of drug effects on its metabolism. |
Features | High sensitivity and wide linear range. Use a 20 µL sample. The detection limit is 2 U/L, linear up to 250 U/L. Homogeneous and simple procedure. A simple “mix-and-measure” procedure allows reliable quantitation of α-glucosidase activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. |
Detection Method | OD405nm |
Sample Type | Biological |
Species | All |
Assay Time | 20 min |
Limit of Detection | 2 U/L |
Expiration Date | 6 months |
Description | α-GLUCOSIDASE hydrolyzes the terminal, non-reducing 1,4-linked α-D-glucose residues with release of α-D-glucose. α-Glucosidase is needed by all animals to hydrolyze maltose to glucose for use as a food. Aberrant activities have been implicated in diseases such as diabetes and Pompe disease. Simple, direct, and automation-ready procedures for measuring α-glucosidase activity are becoming popular in Research and Drug Discovery. CD α-Glucosidase Assay Kit is designed to measure α-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-α-D-glucopyranoside that is hydrolyzed specifically by α-glucosidase into a yellow-colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity. |
Shipping Conditions | Shipping: RT |
Storage | Storage: -20°C upon receipt |
Please note that all services are for research use only. Not intended for any clinical use.
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