CD β-Glucuronidase Assay Kit

Catalog Number: AKBA226

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$1257

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Specifications

Product Information
Product Name	CD β-Glucuronidase Assay Kit
Applications	For quantitative determination of β-glucuronidase enzyme activity in biological samples, e.g. blood, tissue and bacterial samples.
Features	Highly Sensitive. The fluorimetric nature of the kit detects 1×10-4 U/L to 8 U/L BG activity in 96 well plate with only 10 µL sample within 30 min. Versatile. This assay has been validated to work with both animal and E. coli derived β-glucuronidase. High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day.
Detection Method	FL365/450nm
Sample Type	Blood, tissue and bacterial samples
Species	All
Assay Time	30 min
Limit of Detection	10-4 U/L
Expiration Date	6 months
Description	BETA-GLUCURONIDASE (BG) is an enzyme which catalyzes the hydrolysis of β-glucuronide bonds. In humans, β-glucuronidase catalyzes the hydrolysis of β-D-glucuronic acids from glycoproteins and mucopolysaccharides such as heparan sulfate. β-glucuronidase activity is important for drug metabolism as drugs are often conjugated to glucuronic acid to make for a more water soluble delivery system, relying on subsequent β-glucuronidase activity to release the active drug. Bilirubin clearance is also facilitated by glucuronidation. GUSB is often used as a reporter gene to track gene expression through β-glucuronidase activity. BioAssay Systems’ fluorimetric BG assay uses a β-D-glucuronide substrate which fluoresces upon hydrolysis with β-glucuronidase and is read at 365/450 nm.
Shipping Conditions	Shipping: RT
Storage	Storage: -20°C upon receipt.

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