CD Glutamate Assay Kit

Catalog Number: AKBA331

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$1317

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Specifications

Product Information
Product Name	CD Glutamate Assay Kit
Applications	For quantitative determination of glutamate (monosodium glutamate or MSG) and evaluation of drug effects on glutamate metabolism.
Features	Sensitive and accurate. The detection limit of 50 µM, linearity up to 2.5 mM glutamate in a 96-well plate assay. Convenient. The procedure involves adding a single working reagent and reading the optical density at time zero and at 30 min at room temperature. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Detection Method	OD565nm
Sample Type	Serum, plasma, urine, cell, tissue, etc
Species	All
Assay Time	30 min
Limit of Detection	50 µM
Expiration Date	6 months
Description	Glutamate is an important chemical in general metabolism. It is also a crucial mammalian neurotransmitter that is believed to be involved in a number of neurological and psychiatric disorders such as lateral sclerosis, lathyrism, autism, and Alzheimer’s disease. Glutamate is also widely used as a flavor enhancer in the food industry. Simple, direct, and automation-ready procedures for measuring glutamate concentration are very desirable. CD glutamate assay kit is based on glutamate dehydrogenase catalyzed oxidation of glutamate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the glutamate concentration in the sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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