Product Information | |
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Product Name | CD Glutamate Assay Kit |
Applications | For quantitative determination of glutamate (monosodium glutamate or MSG) and evaluation of drug effects on glutamate metabolism. |
Features | Sensitive and accurate. The detection limit of 50 µM, linearity up to 2.5 mM glutamate in a 96-well plate assay. Convenient. The procedure involves adding a single working reagent and reading the optical density at time zero and at 30 min at room temperature. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. |
Detection Method | OD565nm |
Sample Type | Serum, plasma, urine, cell, tissue, etc |
Species | All |
Assay Time | 30 min |
Limit of Detection | 50 µM |
Expiration Date | 6 months |
Description | Glutamate is an important chemical in general metabolism. It is also a crucial mammalian neurotransmitter that is believed to be involved in a number of neurological and psychiatric disorders such as lateral sclerosis, lathyrism, autism, and Alzheimer’s disease. Glutamate is also widely used as a flavor enhancer in the food industry. Simple, direct, and automation-ready procedures for measuring glutamate concentration are very desirable. BioAssay Systems EnzyChrom™ glutamate assay kit is based on glutamate dehydrogenase catalyzed oxidation of glutamate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the glutamate concentration in the sample. |
Shipping Conditions | Shipping: On Ice |
Storage | Storage: -20°C upon receipt |
Please note that all services are for research use only. Not intended for any clinical use.
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