Product Information | |
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Product Name | CD Glutamate Dehydrogenase Assay Kit |
Applications | For quantitative determination of glutamate dehydrogenase activity. |
Features | Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. |
Detection Method | OD565nm |
Sample Type | Serum, plasma, cell, tissue, agriculture etc |
Species | All |
Assay Time | 30 min |
Limit of Detection | 0.1 U/L |
Expiration Date | 6 months |
Description | GLUTAMATE DEHYDROGENASE (GLDH) is an enzyme which catalyzes the interconversion of glutamate and a-ketoglutarate. Elevated blood serum GLDH levels indicate liver damage; thus, GLDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. Transgenic plants expressing microbial GLDHs are improved in tolerance to herbicide, water deficit, and pathogen infections. BioAssay Systems’ non-radioactive, colorimetric GLDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity. |
Shipping Conditions | Shipping: RT |
Storage | Storage: -20°C upon receipt |
Please note that all services are for research use only. Not intended for any clinical use.
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