Product Information | |
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Product Name | CD Glutamine Assay Kit |
Applications | For quantitative determination of glutamine and evaluation of drug effects on glutamine metabolism. |
Features | Sensitive and accurate. Use a 20 µL sample. Linear detection range 0.023 – 2 mM glutamine in a 96-well plate assay. Convenient. The procedure involves adding a single working reagent, incubation for 40 min at room temperature, adding a stop reagent, and reading the optical density. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. |
Detection Method | OD565nm |
Sample Type | Serum, plasma, urine, cell, tissue, etc |
Species | All |
Assay Time | 40 min |
Limit of Detection | 23 µM |
Expiration Date | 6 months |
Description | Glutamine is an amino acid synthesized in the muscle that plays major roles in protein synthesis, acid-base balance, and anabolic processes and is utilized for cellular energy and as a carbon source. It is used in the treatment of injury, trauma, and burns, and also as a supplement for muscle growth and post-surgery healing. Simple, direct, and automation-ready procedures for measuring glutamine concentration are very desirable. CD glutamine assay kit is based on the hydrolysis of glutamine to glutamate and colorimetric determination of the product. The intensity of the product color, measured at 565 nm, is proportional to the glutamine concentration in the sample. |
Shipping Conditions | Shipping: On Ice |
Storage | Storage: -20°C upon receipt |
Please note that all services are for research use only. Not intended for any clinical use.
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