CD Glutathione (GSH) Assay Kit

Catalog Number: AKBA503

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$1347

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Specifications

Product Information
Product Name	CD Glutathione (GSH) Assay Kit
Applications	For quantitative determination of reduced glutathione (GSH) and evaluation of drug effects on glutathione metabolism.
Features	Sensitive and accurate. Linear detection range 0.4 – 100 µM in 96-well plate. Simple and convenient. The procedure involves mixing the DTNB Reagent with sample, removing protein precipitates for proteinaceous samples, adding a second Reagent and reading the optical density. Low interference. Amino acids and common buffers do not interfere.
Detection Method	OD412nm
Sample Type	Whole blood, plasma, serum, urine, tissue and cell extracts
Species	All
Assay Time	30 min
Limit of Detection	12 µg/dL (0.4 µM)
Expiration Date	12 months
Description	Glutathione is a tripeptide of glycine, glutamic acid and cysteine. In the red blood cell, the reduced form of glutathione is vital in maintaining hemoglobin in a reduced state and hence protecting the cells from oxidative damage. Glutathione is involved in detoxification of hydrogen peroxide through glutathione oxidase. Low levels of glutathione are found in deficiencies of key enzymes involved in glutathione metabolism, such as glucose-6-phosphate dehydrogenase, glutathione synthase and glutathione reductase. Simple, direct and automation-ready procedures for measuring reduced glutathione are becoming popular in Research and Drug Discovery. CD Glutathione Assay Kit is designed to accurately measure reduced glutathione in biological samples. The improved 5,5’-dithiobis(2-nitrobenzoic acid (DTNB) method combines deproteination and detection (Reagent A) into one reagent. DTNB reacts with reduced glutathione to form a yellow product. The optical density, measured at 412 nm, is directly proportional to glutathione concentration in the sample. The optimized formulation has a long shelf life and is completely free of interference due to sample turbidity.
Shipping Conditions	Shipping: RT
Storage	Storage: 4°C upon receipt

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