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(AKBA233)CD Glutathione S-transferase Assay Kit

CD Glutathione S-transferase Assay Kit

Catalog Number: AKBA233

Price:
$1017
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Specifications

Product Information
Product Name CD Glutathione S-transferase Assay Kit
Applications For quantitative determination of glutathione S-transferase activity and evaluation of drug modulators.
Features Fast and sensitive. Linear detection range (20 µL sample): 2 to 80 U/L for 10 min reaction at 25°C.
Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method OD340nm
Sample Type Biological samples (e.g. cell lysates, tissues, etc.)
Species All
Assay Time 10 min
Limit of Detection 2 U/L
Expiration Date 6 months
Description GLUTATHIONE TRANSFERASE (GST; EC 2.5.1.18) is a multifunctional enzyme that plays an important role in cellular detoxification. GST protects cells against foreign compounds such as carcinogens and drugs by catalyzing the attachment of glutathione to the compound’s electrophilic and/or hydrophobic sites.CD Glutathione S-transferase assay kit is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1-chloro-2,4-dinitrobenzene). The GST-catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected spectrophotometrically at 340 nm. The rate of increase in absorbance at 340 nm is directly proportional to the GST activity in the sample.
Shipping Conditions Shipping: RT
Storage Storage: -20°C upon receipt

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