CD Glutathione S-transferase Assay Kit

Catalog Number: AKBA233

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$1017

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Specifications

Product Information
Product Name	CD Glutathione S-transferase Assay Kit
Applications	For quantitative determination of glutathione S-transferase activity and evaluation of drug modulators.
Features	Fast and sensitive. Linear detection range (20 µL sample): 2 to 80 U/L for 10 min reaction at 25°C. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD340nm
Sample Type	Biological samples (e.g. cell lysates, tissues, etc.)
Species	All
Assay Time	10 min
Limit of Detection	2 U/L
Expiration Date	6 months
Description	GLUTATHIONE TRANSFERASE (GST; EC 2.5.1.18) is a multifunctional enzyme that plays an important role in cellular detoxification. GST protects cells against foreign compounds such as carcinogens and drugs by catalyzing the attachment of glutathione to the compound’s electrophilic and/or hydrophobic sites.BioAssay Systems Glutathione S-transferase assay kit is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1-chloro-2,4-dinitrobenzene). The GST-catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected spectrophotometrically at 340 nm. The rate of increase in absorbance at 340 nm is directly proportional to the GST activity in the sample.
Shipping Conditions	Shipping: RT
Storage	Storage: -20°C upon receipt

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