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(AKBA027)CD Glycerol Assay Kit

CD Glycerol Assay Kit

Catalog Number: AKBA027

Price:
$1287
Specifications Downloads Related products

Specifications

Product Information
Product Name CD Glycerol Assay Kit
Applications For quantitative determination of glycerol and evaluation of drug effects on glycerol metabolism.
Features Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 10 to 1000 µM (92 µg/dL to 9.2 mg/dL) glycerol for colorimetric assays and 2 to 50 µM for fluorimetric assays.
Simple and convenient. The procedure involves the addition of a single working reagent and incubation for 20 min at room temperature, compatible with HTS assays.
Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.
Detection Method OD570nm, or FL530/585nm
Sample Type Biological, food, beverage, etc
Species All
Assay Time 20 min
Limit of Detection 2 µM
Expiration Date 12 months
Description Glycerol [Glycerin or Glycerine, C3H5 (OH) 3] is widely used in foods, beverages, and pharmaceutical formulations. It is also a main by-product of biodiesel production. Simple, direct, and automation-ready procedures for measuring glycerol concentrations find wide applications. CD glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase, and color reactions in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to glycerol concentration in the sample.
Shipping Conditions Shipping: On Ice
Storage Storage: -20°C upon receipt

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