CD Glyoxalase I Assay Kit

Catalog Number: AKBA234

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$1287

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Specifications

Product Information
Product Name	CD Glyoxalase I Assay Kit
Applications	For quantitative determination of glyoxalase enzyme activity.
Features	Fast and sensitive. Detection limit of 4 U/L GLO-1 activity. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD240nm (Use a UV plate, e.g. 96-well UV plate, cat# P96UV)
Sample Type	Enzyme preparations or biological samples
Species	All
Assay Time	30 min
Limit of Detection	4 U/L
Expiration Date	12 months
Description	GLYOXALASE I (GLO-1), a lactoylglutathione lyase also known as methylglyoxalase, aldoketomutase, ketone-aldehyde mutase, and (R)-S-lactoylglutathione methylglyoxal-lyase, is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methylglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. Glyoxalase I is a target for the development of pharmaceuticals against bacteria, protozoans and human cancer. Simple, direct and automation-ready procedures for measuring GLO-1 activity in biological samples are highly desirable in research and drug discovery. CD Glyoxalase I assay kit provides a sensitive and convenient method for GLO-1 activity determination. The method involves monitoring the increase in the product of the GLO-1 reaction, S-lactoylglutathione, by measuring the change in absorbance at 240 nm.
Shipping Conditions	Shipping: RT
Storage	Storage: -20°C upon receipt

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