CD GSH/GSSG Assay Kit

Catalog Number: AKBA029

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$1077

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Specifications

Product Information
Product Name	CD GSH/GSSG Assay Kit
Applications	For quantitative determination of reduced/oxidized glutathione (GSH/GSSG) and evaluation of drug effects on their metabolism.
Features	Sensitive and accurate. Linear detection range 0.01-3 µM GSH equivalents with a detection limit of 10 nM GSH equivalents.
Detection Method	OD412nm
Sample Type	Whole blood, plasma, serum, urine, tissue, and cell extracts
Species	All
Assay Time	20 min
Limit of Detection	10 nM
Expiration Date	6 months
Description	Glutathione, a tripeptide of glycine, glutamic acid, and cysteine, is one of the key antioxidants involved in protecting cells from damage by reactive oxygen species. Glutathione (GSH) reduces disulfide bonds in cytoplasmic proteins to cysteines, in which it is converted to its oxidized form GSSG. CD GSH/GSSG Assay Kit is designed to accurately measure total, reduced, and oxidized glutathione in biological samples using an enzymatic method that utilizes Ellman’s Reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change in the optical density, measured at 412 nm, is directly proportional to glutathione concentration in the sample. This kit can also be used to measure oxidized (GSSG) by using a specific protocol that first scavenges all GSH with 1-methyl-2-vinylpyridinium triflate.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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