CD HDL and LDL/VLDL Assay Kit

Catalog Number: AKBA030

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$1038

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Specifications

Product Information
Product Name	CD HDL and LDL/VLDL Assay Kit
Applications	For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.
Features	Sensitive and accurate. Requires only 20 µL serum sample. The detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in 96-well plate assay. Convenient. Room temperature assay. No 37°C heater is needed. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD340nm
Sample Type	Serum
Species	All
Assay Time	60 min
Limit of Detection	5 mg/dL
Expiration Date	6 months
Description	Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors of coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to a higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined but can be changed by medications, food choices, and other factors. Simple, direct, and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable. CD HDL and LDL/VLDL quantification kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using cholesterol esterase/cholesterol dehydrogenase reagent. In this reaction, NAD is reduced to NADH. The optical density of the formed NADH at 340 nm is directly proportionate to the cholesterol concentration in the sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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