CD Hexokinase Assay Kit

Catalog Number: AKBA031

Price:

$1377

- +

ADD TO CART

Specifications Downloads Related products

Specifications

Product Information
Product Name	CD Hexokinase Assay Kit
Applications	Hexokinase activity determination in biological samples (e.g. microorganisms, plasma, serum, cultured cells, tissue and culture media.)
Features	Fast and sensitive. Linear detection range (20 μL sample): 0.22 to 100 U/L for 15 min reaction. Convenient. Homogeneous “mix-incubate-measure” type assay. High-throughput. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD565nm
Sample Type	Biological samples (e.g. microorganisms, plasma, serum, cultured cells, tissue, and culture media.)
Species	All
Assay Time	15 min
Limit of Detection	0.22 U/L
Expiration Date	6 months
Description	HEXOKINASE (E.C. 2.7.1.1) is a mitochondrial and cytosolic enzyme and the first committed step in the glycolysis pathway. Hexose sugars, mainly glucose, are phosphorylated using ATP to form glucose-6-phosphate. Hexokinases are implicated in a myriad of human diseases, including cancer and diabetes. BioAssay Systems’ non-radioactive, colorimetric Hexokinase assay involves a stepwise reaction based on the reduction of the tetrazolium salt MTT in an NADPH-coupled enzymatic reaction to a colored product which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt.

Downloads

Datasheet

Please note that all services are for research use only. Not intended for any clinical use.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.