CD Human/Mouse AKT1(S473) Phosphorylation ELISA Kit

Catalog Number: AKBA156

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$1038

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Product Information
Product Name	CD Human/Mouse AKT1(S473) Phosphorylation ELISA Kit
Applications	For determination of AKT1(S473) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc.
Features	New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis is necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Detection Method	Cell-Based ELISA (FL530/585 nm, 360/450 nm)
Sample Type	Cells
Species	human, rat and mouse
Assay Time	Assay takes 6.5 hrs, hands-on time 2.5 hrs
Expiration Date	6 months
Description	PROTEIN PHOSPHORYLATION plays key role in regulating protein activity within cells. A large number of protein kinases and phosphatases are known to be involved in a variety of signal transduction pathways. A rapid assay capable of monitoring such signal transduction pathways, target protein phosphorylation status and drug effects are highly desirable in life science research and drug discovery.AKT1 is a serine-threonine protein kinase that is activated through phosphatidylinositol 3-kinase by platelet-derived growth factor. In the developing nervous system, AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the AKT1. BioAssay Systems’ AKT1(S473) Phosphorylation ELISA Kit provides a rapid and sensitive assay for measuring the phosphorylated protein pAKT1(S473) in cultured cells and normalizes the level of phosphorylation to the total protein content in the same well. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study the underlying signal pathway and the effects of inhibitors, siRNA or activators on the pathway. In this assay, cells grown in a 96-well plate are fixed and permeabilized. The phosphorylation of AKT1(S473) is measured using a specific primary antibody followed by an HRP-conjugated secondary antibody with a fluorogenic substrate, and a fluorescent reagent that measures the total protein in the same well.Haven’t Found the ELISA Kit for Your TargetWe offer two DIY kits: EFC1M-100 for mouse antibodies and EFC2R-100 for rabbit antibodies. All that is required is a primary antibody. If you prefer, we can develop the assay for you. Please email or call 1-510-782-9988 x 2 to request assay service.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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Please note that all services are for research use only. Not intended for any clinical use.

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