Product Information | |
---|---|
Product Name | CD Inosine Assay Kit |
Applications | For determination of inosine concentration in cell lysate, serum, and other biological samples. |
Features | Sensitive and accurate. Use as little as 20 μL of sample. Linear detection range in 96-well plate for 30-minute incubation with 1 to 25 μM. Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature. Fast and high-throughput. Assays using 96-well plates and liquid handling system could allow simultaneous processing tens of thousands of samples per day. |
Detection Method | FL530/585nm |
Sample Type | Biological samples |
Species | All |
Assay Time | 30 min |
Limit of Detection | 1 μM |
Expiration Date | 6 months |
Description | INOSINE is a purine nucleoside formed from Ribose and Hypoxanthine that can be found in most animal tissues and fluids. Inosine is most often found in tRNAs and is important for proper translation of the genetic code. It may play a role in cancer immunometabolism. It is degraded via the purine degradation pathway, first by purine nucleoside phosphorylase to hypoxanthine, then to xanthine by xanthine oxidase and then finally to uric acid by further action of xanthine oxidase.Simple, direct and high-throughput assays for measuring inosine find wide applications in research and drug discovery. BioAssay Systems’ inosine assay kit uses a single Working Reagent that combines the enzyme reactions and color reaction in one step. The change in fluorescence intensity at λex/em = 530/585 nm is directly proportional to inosine concentration in the sample. |
Storage | Storage: -20°C upon receipt. |
Please note that all services are for research use only. Not intended for any clinical use.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
There is no product in your cart. |
CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.