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(AKBA051)CD Inosine Assay Kit

CD Inosine Assay Kit

Catalog Number: AKBA051

Price:
$1287
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Specifications

Product Information
Product Name CD Inosine Assay Kit
Applications For determination of inosine concentration in cell lysate, serum, and other biological samples.
Features Sensitive and accurate. Use as little as 20 μL of sample. Linear detection range in 96-well plate for 30-minute incubation with 1 to 25 μM.
Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature.
Fast and high-throughput. Assays using 96-well plates and liquid handling system could allow simultaneous processing tens of thousands of samples per day.
Detection Method FL530/585nm
Sample Type Biological samples
Species All
Assay Time 30 min
Limit of Detection 1 μM
Expiration Date 6 months
Description INOSINE is a purine nucleoside formed from Ribose and Hypoxanthine that can be found in most animal tissues and fluids. Inosine is most often found in tRNAs and is important for proper translation of the genetic code. It may play a role in cancer immunometabolism. It is degraded via the purine degradation pathway, first by purine nucleoside phosphorylase to hypoxanthine, then to xanthine by xanthine oxidase and then finally to uric acid by further action of xanthine oxidase.Simple, direct and high-throughput assays for measuring inosine find wide applications in research and drug discovery. BioAssay Systems’ inosine assay kit uses a single Working Reagent that combines the enzyme reactions and color reaction in one step. The change in fluorescence intensity at λex/em = 530/585 nm is directly proportional to inosine concentration in the sample.
Storage Storage: -20°C upon receipt.

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