CD Iron Assay Kit

Catalog Number: AKBA566

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$1317

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Specifications

Product Information
Product Name	CD Iron Assay Kit
Applications	For quantitative determination of iron ions Fe3+ and/or Fe2+ and evaluation of drug effects on iron metabolism.
Features	Sensitive and accurate. Linear detection range 27 µg/dL (4.8 µM) to 1,000 µg/dL (179 µM) iron in a 96-well plate assay. Simple and high-throughput. The procedure involves the addition of a single working reagent and incubation for 40 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced the reagent and signal stability. Cuvette or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples.
Detection Method	OD590nm
Sample Type	Biological (e.g. serum) and environmental samples
Species	All
Assay Time	30 min
Limit of Detection	27 µg/dL (4.8 µM)
Expiration Date	12 months
Description	Iron level in blood is a reliable diagnostic indicator of various disease states. Increased levels of iron concentration in blood are associated with blood loss, increased destruction of red blood cells (e.g. hemorrhage) or decreased blood cell survival, acute hepatitis, certain sideroachrestic anemias, ingestion of iron-rich diets, defects in iron storage (e.g. pernicious anemia). Decreased levels of blood iron may result from insufficient iron ingestion from diets, chronic blood loss pathologies, or increased demand for iron storage during normal pregnancy. Simple, direct, and automation-ready procedures for measuring iron concentrations find wide applications in research, drug discovery, and environmental monitoring. CD iron assay kit is designed to measure total iron directly in serum without any pretreatment. The improved method utilizes a chromogen that forms a blue-colored complex specifically with Fe2+. Fe3+ in the sample is reduced to Fe2+, thus allowing the assay for total iron concentration. The intensity of the color, measured at 590nm, is directly proportional to the iron concentration in the sample.
Shipping Conditions	Shipping: RT
Storage	Storage: 4°C upon receipt

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