CD Isocitrate Assay Kit

Catalog Number: AKBA162

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$1257

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Specifications

Product Information
Product Name	CD Isocitrate Assay Kit
Applications	For quantitative fluorimetric determination of isocitrate and evaluation of drug effects on its metabolism.
Features	Fast and sensitive. Linear detection range (20 µL sample): 0.6 to 500 µM for 10 min reaction. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	FL530/585 nm
Sample Type	Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
Species	All
Assay Time	10 min
Limit of Detection	0.6 µM
Expiration Date	6 months
Description	ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. BioAssay Systems’ isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the isocitrate concentration in the sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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