CD Ketone Body Assay Kit

Catalog Number: AKBA032

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$1467

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Specifications

Product Information
Product Name	CD Ketone Body Assay Kit
Applications	For quantitative determination of ketone body (acetoacetate and 3-hydroxybutyrate) and evaluation of drug effects on its metabolism.
Features	Sensitive and accurate. Uses a 10 µL sample. Linear detection range of 0.12 to 8 mM for each ketone body in 96-well plate assay. Convenient. The procedure involves adding a single working reagent and reading the optical density at room temperature. High-throughput. Can be automated as a high-throughput 96-well plate assay for many samples per day.
Detection Method	OD340nm
Sample Type	Serum, plasma, urine etc
Species	All
Assay Time	30 min
Limit of Detection	0.12 mM
Expiration Date	6 months
Description	Ketone bodies (acetoacetic acid and 3-hydroxybutyric acid) are produced in the liver mainly from oxidation of fatty acids and are normally present at low concentrations in urine and blood. Increased ketone concentrations in the blood may lead to metabolic acidosis, which has been associated with diabetes, childhood hypo-glycemia, growth hormone deficiency, alcohol or salicylate intoxication, and inborn errors of metabolism. Simple, direct, and automation-ready procedures for measuring acetoacetic acid (AcAc) and 3-hydroxybutyric acid (BOH) are very desirable. CD ketone body assay is based on 3-hydroxybutyrate dehydrogenase catalyzed reactions, in which the change in NADH absorbance, measured at 340nm, is directly related to the AcAc and BOH concentrations,
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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