CD Kinase Assay Kit

Catalog Number: AKBA200

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$927

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Specifications

Product Information
Product Name	CD Kinase Assay Kit
Applications	Rapid homogenous assay for protein kinase activity and high-throughput screen for kinase inhibitors.
Features	Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.01 U/L kinase can be quantified. Homogeneous and convenient. “Mix-incubate-measure” type assay. The whole assay involves adding a single working reagent and incubation for 10 min at room temperature. Robust and amenable to HTS: Assay can tolerate up to 300 µM ATP and 10% dimethylsulfoxide (DMSO). Z factors of > 0.6 are routinely observed in 96/384-well plates. Can be readily automated on HTS liquid handling systems for tens of thousands of assays per day.
Detection Method	FL530/585nm
Sample Type	All kinases
Species	All
Assay Time	10 min
Limit of Detection	0.01 U/L
Expiration Date	6 months
Description	Kinases, also known as phosphotransferases, constitute a large family of enzymes that transfer phosphate groups from the high-energy donor molecule ATP to their specific substrates. Kinases are known to regulate the majority of cellular processes. The largest group of this family is the protein kinases. So far, 518 different kinases have been identified in humans, and up to 30% of human proteins are modified by these kinases. The enormous diversity and their key role in cellular signaling make them ideal targets for drug development. BioAssay Systems’ Kinase Assay Kit provides a simple and rapid method for assaying kinase activity and high-throughput screening for kinase inhibitors. This homogeneous microplate-based assay involves incubating the kinase with a single working reagent, in which ADP is enzymatically converted to ATP and pyruvate, which is quantified using a fluorimetric (530nm/590nm) assay method.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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