CD α-L-Fucosidase Assay Kit

Catalog Number: AKBA055

Price:

$1347

- +

ADD TO CART

Specifications Downloads Related products

Specifications

Product Information
Product Name	CD α-L-Fucosidase Assay Kit
Applications	For quantitative determination of α-L-Fucosidase activity in biological samples.
Features	High sensitivity and wide linear range. Linear detection range (10 µL sample): 1 to 100 U/L. Homogeneous and simple procedure. A simple add-mix-read procedure allows reliable quantitation of fucosidase activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Can be readily automated to measure thousands of samples per day.
Detection Method	OD405nm
Sample Type	Plasma, serum, tissue, cell lysate, etc
Species	All
Assay Time	20 min
Limit of Detection	1 U/L
Expiration Date	6 months
Description	α-L-FUCOSIDASE (AFU) is an enzyme coded by the FUCA1 gene in humans and catalyzes the breakdown of L-Fucose. A genetic deficiency in this enzyme results in a neurovisceral storage disease, fucosidosis, which is characterized by the accumulation of fucose. Low serum activity of fucosidase has also been linked to ovarian carcinoma. Elevated fucosidase serum activity has been observed in patients with diabetes, hyperthyroidism, cirrhosis, and hepatitis. Increased activity has been associated with lung, breast, stomach, ovary, uterus, and liver carcinomas.BioAssay System’s non-radioactive, colorimetric AFU assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after the addition of the stop reagent. The increase in absorbance at 405 nm after the addition of the stop reagent is directly proportional to the enzyme activity.
Shipping Conditions	Shipping: RT
Storage	Storage: 4°C upon receipt

Downloads

Datasheet

Please note that all services are for research use only. Not intended for any clinical use.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.