CD Lactose Assay Kit

Catalog Number: AKBA035

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$1317

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Specifications

Product Information
Product Name	CD Lactose Assay Kit
Applications	For quantitative determination of lactose and evaluation of drug effects on lactose metabolism.
Features	Sensitive. Use as little as 20 µL samples. Linear detection range in 96-well plate: 17 to 2000 µM lactose for colorimetric assays and 6 to 100 µM for fluorimetric assays. Convenient and high-throughput. Robust and homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD570nm, or FL530/585nm
Sample Type	Biological (milk), food and agriculture, etc
Species	All
Assay Time	30 min
Limit of Detection	6 µM
Expiration Date	6 months
Description	Lactose (C12H22O11), also called milk sugar, is a disaccharide that consists of β-D-galactose and α/β-D-glucose through a β1-4 glycosidic linkage. Lactose is the major sugar and makes up 2–8% of milk. Simple, direct, and high-throughput assays for lactose determination find wide applications. CD assay uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the lactose concentration in the sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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