CD LDH Cytotoxicity Assay Kit

Catalog Number: AKBA291

Price:

$387

- +

ADD TO CART

Specifications Downloads Related products

Specifications

Product Information
Product Name	CD LDH Cytotoxicity Assay Kit
Applications	For rapid quantitative determination of cytotoxicity based on lactate dehydrogenase released into the cell culture medium. Evaluation of toxic compounds, toxins, detergents, environmental pollutants and physical treatment on cell lysis.
Features	Safe. Non-radioactive assay (cf. chromium release assay). Fast. The whole procedure takes 20 min. Robust and amenable to HTS. Single reagent, “mix-incubate-measure” type assay. High-throughput assay in 96-well plates allows the simultaneous processing of tens of thousands of samples per day.
Detection Method	OD500nm
Sample Type	Cell culture
Species	All
Assay Time	20 min
Expiration Date	12 months
Description	Lactate dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. Cytotoxic compounds often compromise cell membrane integrity by inducing apoptosis or necrosis. LDH is a stable cytosolic enzyme that upon membrane damage is released into the cellular environment. Therefore, LDH is often measured to evaluate the presence of tissue or cell damage. The colorimetric LDH release assay is a simple and robust method to assess cytotoxic effects on cells by measuring the activity of LDH in the cell culture supernatant. The assay is based on the reduction of a tetrazolium salt to a formazan dye.
Shipping Conditions	Shipping: RT
Storage	Storage: -20°C upon receipt

Downloads

Datasheet

Please note that all services are for research use only. Not intended for any clinical use.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.