Product Information | |
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Product Name | CD α-Mannosidase Assay Kit |
Applications | For quantitative determination of α-mannosidase enzyme activity. |
Features | Fast and sensitive. Linear detection range (10 µL sample): 1 to 250 U/L for a 10-minute reaction. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. |
Detection Method | OD405nm |
Sample Type | Biological samples e.g. plasma, serum, tissue, and culture media |
Species | All |
Assay Time | 10 min |
Limit of Detection | 1 U/L |
Expiration Date | 6 months |
Description | α-MANNOSIDASE (AMA) is an enzyme that catalyzes the cleavage of the α form of mannose. α-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes α-mannosidosis and leads to deterioration of the central nervous system in children. BioAssay Systems’ non-radioactive, colorimetric AMA assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after the addition of the stop reagent. The increase in absorbance at 405 nm after the addition of the stop reagent is directly proportional to the enzyme activity. |
Shipping Conditions | Shipping: RT |
Storage | Storage: 4°C upon receipt |
Please note that all services are for research use only. Not intended for any clinical use.
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