CD Myeloperoxidase Assay Kit

Catalog Number: AKBA222

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$1317

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Specifications

Product Information
Product Name	CD Myeloperoxidase Assay Kit
Applications	For quantitative determination of myeloperoxidase enzyme activity and evaluation of drug modulators.
Features	Fast and sensitive. Linear detection range (20 µL sample): 0.0025 to 2 U/L for 10 min reaction at 25°C. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	FL530/585nm
Sample Type	Cell lysates, tissues, etc
Species	All
Assay Time	Approximately 30 min
Limit of Detection	0.0025 U/L
Expiration Date	6 months
Description	MYELOPEROXIDASE (MPO; EC 1.11.2.2) is a peroxidase enzyme and can be found in neutrophils, monocytes, and some soft tissue macrophages. MPO has the ability to use chloride as a cosubstrate with hydrogen peroxide to generate hypochlorous acid, a powerful antimicrobial agent produced by neutrophils. However, excessive production of hypochlorous acid can lead to oxidative stress and tissue damage. Inflammation may also result when MPO oxidizes various substances such as phenols and anilines. Studies show that increased MPO levels may increase the risk of myocardial infarction and cardiovascular disease.CD EnzyFluoTM myeloperoxidase (MPO) assay kit is based on the MPO enzyme reaction with hydrogen peroxide (H2O2) which oxidizes the dye reagent to a highly fluorescent product. The fluorescence intensity of this product, measured at &labda; ex/em = 530/585 nm, is proportional to the total peroxidation activity in the sample. The provided MPO inhibitor is used to suppress peroxidase activity due to MPO in order to differentiate other peroxidase activities that may be present in the samples.Related Categories:Oxidative-Stress.htm” target=_blank>Oxidative Stress, Enzyme-Activity-Assays.htm” target=_blank>Enzyme Activity Assays
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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