Product Information | |
---|---|
Product Name | CD NAD/NADH Assay Kit |
Applications | For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism. |
Features | Sensitive and accurate. Detection limit of 0.02 µM and linearity up to 1 µM NAD+/NADH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 min. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. |
Detection Method | F530/585nm |
Sample Type | Cell, tissue extracts etc Direct NAD/NADH Assays in 96-Well PlateNEW!!! |
Species | All |
Assay Time | 10 min |
Limit of Detection | 0.02 µM |
Expiration Date | 6 months |
Description | Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. CD NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH and is a convenient method to measure NAD, NADH and their ratio.Direct NAD/NADH Assays in 96-Well PlateNEW!!! |
Shipping Conditions | Shipping: On Ice |
Storage | Storage: -20°C upon receipt |
Please note that all services are for research use only. Not intended for any clinical use.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
There is no product in your cart. |
CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.