CD NAD/NADH Assay Kit

Catalog Number: AKBA164

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Specifications

Product Information
Product Name	CD NAD/NADH Assay Kit
Applications	For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism.
Features	Sensitive and accurate. Detection limit of 0.02 µM and linearity up to 1 µM NAD+/NADH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 10 min. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Detection Method	F530/585nm
Sample Type	Cell, tissue extracts etc Direct NAD/NADH Assays in 96-Well PlateNEW!!!
Species	All
Assay Time	10 min
Limit of Detection	0.02 µM
Expiration Date	6 months
Description	Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. CD NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH with minimal interference (<1%) by NADP+/NADPH and is a convenient method to measure NAD, NADH and their ratio.Direct NAD/NADH Assays in 96-Well PlateNEW!!!
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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