CD NAD/NADH Assay Kit

Catalog Number: AKBA140

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$1467

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Specifications

Product Information
Product Name	CD NAD/NADH Assay Kit
Applications	For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism.
Features	Sensitive and accurate. The detection limit of 0.05 µM and linearity up to 10 µM NAD+/NADH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 15 min at room temperature. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Detection Method	OD565nm
Sample Type	Cell or tissue extracts
Species	All
Assay Time	15 min
Limit of Detection	0.05 µM
Expiration Date	6 months
Description	Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and the redox state of cells or tissue. Simple, direct, and automation-ready procedures for measuring NAD+/NADH concentration are very desirable. CD NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH and with minimal interference (<1%) by NADP+/NADPH. Our assay is a convenient method to measure NAD, NADH, and their ratio.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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