CD NADP+/NADPH Assay Kit

Catalog Number: AKBA037

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$1467

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Specifications

Product Information
Product Name	CD NADP+/NADPH Assay Kit
Applications	For quantitative determination of NADP+/NADPH concentrations and ratios in cell or tissue extracts.
Features	Sensitive and accurate. Detection limit 0.1 µM, linearity up to 10 µM NADP+/NADPH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 30 min at room temperature. No 37°C heater is required. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Detection Method	Colorimetric Determination at 565 nm
Sample Type	Cells, Tissue
Species	human, mouse, rat, and more
Assay Time	30 min
Limit of Detection	0.1 µM
Expiration Date	6 months
Description	Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and the redox state of cells or tissue.Simple, direct, and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable. BioAssay Systems’ EnzyChromTM NADP+ /NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+/NADPH concentration in the sample. This assay is highly specific for NADP+/NADPH and is not interfered with by NAD+/NADH. Our assay is a convenient method to measure NADP, NADPH, and their ratio.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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