Product Information | |
---|---|
Product Name | CD NADP/NADPH Assay Kit |
Applications | For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism (This product is a replacement for the discontinued product EFNP-100). |
Features | Sensitive and accurate. The detection limit of 0.01 µM and linearity up to 1 µM NADP+/NADPH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent and reading the fluorescence at time zero and 30 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. |
Detection Method | FL530/585nm |
Sample Type | Cell, tissue extracts, etc |
Species | All |
Assay Time | 30 min |
Limit of Detection | 0.01 µM |
Expiration Date | 6 months |
Description | Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and the redox state of cells or tissue. Simple, direct, and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable. BioAssay Systems EnzyFluo™ NADP+/NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the NADP+/NADPH concentration in the sample. This assay is highly specific for NADP+/NADPH and with minimal interference (<1%) by NAD+/NADH. Our assay is a convenient method to measure NADP+, NADPH, and their ratio. |
Shipping Conditions | Shipping: On Ice |
Storage | Storage: -20°C upon receipt |
Please note that all services are for research use only. Not intended for any clinical use.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
There is no product in your cart. |
CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.