CD NADP/NADPH Assay Kit

Catalog Number: AKBA379

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$1467

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Specifications

Product Information
Product Name	CD NADP/NADPH Assay Kit
Applications	For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism (This product is a replacement for the discontinued product EFNP-100).
Features	Sensitive and accurate. The detection limit of 0.01 µM and linearity up to 1 µM NADP+/NADPH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent and reading the fluorescence at time zero and 30 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Detection Method	FL530/585nm
Sample Type	Cell, tissue extracts, etc
Species	All
Assay Time	30 min
Limit of Detection	0.01 µM
Expiration Date	6 months
Description	Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and the redox state of cells or tissue. Simple, direct, and automation-ready procedures for measuring NADP+/NADPH concentration are very desirable. CD NADP+/NADPH assay kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the NADP+/NADPH concentration in the sample. This assay is highly specific for NADP+/NADPH and with minimal interference (<1%) by NAD+/NADH. Our assay is a convenient method to measure NADP+, NADPH, and their ratio.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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