CD Nitric Oxide Assay Kit

Catalog Number: AKBA505

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$1017

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Specifications

Product Information
Product Name	CD Nitric Oxide Assay Kit
Applications	For quantitative determination of nitric oxide (nitrate/nitrite) and evaluation of drug effects on its metabolism.
Features	Sensitive and accurate. Detection range 0.6 – 200 µM in a 96-well plate. Rapid and reliable. Using an optimized VCl3 reagent, the time required for the reduction of nitrate (NO3–) to nitrite (NO2–) is reduced to 10 min at 60°C. Simple and high-throughput. The procedure involves mixing the sample with three reagents, incubation for 10 min at 60°C, and reading the optical density. Can be readily automated to measure thousands of samples per day.
Detection Method	OD540nm
Sample Type	Plasma, serum, urine, tissue/cells, and foods
Species	All
Assay Time	40 min
Limit of Detection	0.6 µM
Expiration Date	6 months
Description	Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO, an oxidation product of arginine by nitric oxide synthase, is involved in host defense and development, activation of regulatory proteins, and direct covalent interaction with functional biomolecules. Simple, direct, and automation-ready procedures for measuring NO are becoming popular in Research and Drug Discovery. Since NO is oxidized to nitrite and nitrate, it is common practice to quantitate total NO2-/NO3- as a measure of NO level. CD Nitric Oxide Assay Kit is designed to accurately measure NO production following the reduction of nitrate to nitrite using improved Griess method. The procedure is simple and the time required for sample pretreatment and assay is reduced to as short as 30 min.
Shipping Conditions	Shipping: RT
Storage	Storage: -20°C upon receipt

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