CD Peroxidase Assay Kit

Catalog Number: AKBA506

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$1047

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Specifications

Product Information
Product Name	CD Peroxidase Assay Kit
Applications	For quantitative determination of peroxidase enzyme activity.
Features	Fast and sensitive. Use as little as 10 µL samples. Linear detection range: colorimetric assays 2 to 50 U/L, fluorimetric assays 0.1 to 5 U/L peroxidase. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD570nm, or FL530/585nm
Sample Type	Biological
Species	All
Assay Time	20 min
Limit of Detection	OD, FL: 4, 0.8 U/L
Expiration Date	12 months
Description	PEROXIDASES (EC number 1.11.1.x) catalyzes the following oxidation-reduction reactions: ROOR + electron donor (2 e-) + 2H+ → ROH + R’OH. For many peroxidases, the optimal substrate is hydrogen peroxide (H2O2), but others are more active with organic hydroperoxides such as lipid peroxides. In the cell, peroxidases destroy toxic hydroxide radicals that are formed as byproducts during aerobic respiration. The peroxidases represent a large family of enzymes that are found in animals (e.g. myeloperoxidase-like enzymes), plants, fungi, and bacteria (cytochrome-c peroxidase-like enzymes such as horseradish peroxidase). Simple, direct, and automation-ready procedures for determining peroxidase activity find wide applications. CD peroxidase assay uses H2O2 and an electron donor dye that forms a pink color during the peroxidase reaction. The optical density (570nm) or fluorescence intensity (λex/em = 530/590nm) is a direct measure of the enzyme activity.duangwatch
Shipping Conditions	Shipping: RT
Storage	Storage: -20°C upon receipt

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