CD Phosphate Assay Kit

Catalog Number: AKBA085

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$1107

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Specifications

Product Information
Product Name	CD Phosphate Assay Kit
Applications	For quantitative determination of phosphate and evaluation of drug effects on phosphate metabolism.
Features	Sensitive and accurate. Linear detection range 0.3 µM (0.0028 mg/dL) to 50 µM (0.47 mg/dL) phosphate in a 96-well plate assay. Simple and high-throughput. The procedure involves the addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced the reagent and signal stability. Assays can be executed in cuvet or 96-well plates. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipids, proteins, and minerals.
Detection Method	OD620nm (malachite green)
Sample Type	Serum, urine, saliva, sweat, tissue culture, food, environment, etc
Species	All
Assay Time	30 min
Limit of Detection	3 µg/dL (0.3 µM)
Expiration Date	12 months
Description	Phosphate (Pi) is one of the most important ion species in nature. Phosphate is present in all biological systems. It is a major constituent in minerals and fertilizers and is a component of industrial wastewater. Thus the accurate determination of phosphate concentration finds numerous applications in pharmacology, biomedical research, clinical chemistry, industrial process monitoring, and environmental monitoring. Simple, direct, and automation-ready procedures for measuring phosphate concentration in biological and environmental samples are becoming popular. BioAssay Systems phosphate assay kit is designed to measure phosphate ions directly in samples without any pretreatment. The improved Malachite Green method utilizes the malachite green dye and molybdate, which forms a stable colored complex specifically with inorganic phosphate. The intensity of the color, measured at 620nm, is directly proportional to the phosphate concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
Shipping Conditions	Shipping: RT
Storage	Storage: 4°C upon receipt

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