CD Phospholipase D Assay Kit

Catalog Number: AKBA041

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$1167

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Specifications

Product Information
Product Name	CD Phospholipase D Assay Kit
Applications	For quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.
Features	Sensitive. Use 10 µL samples. Detection range: colorimetric assay 0.06 – 10 U/L, fluorimetric assay 0.04 – 1 U/L. Simple and High-throughput: the assay involves the addition of a single working reagent and can be readily adapted to high-throughput assays for drug screening.
Detection Method	OD570nm, or FL530/585nm
Sample Type	Biological
Species	All
Assay Time	30 min
Limit of Detection	0.04 U/L
Expiration Date	6 months
Description	Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers. CD method provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and an H2O2-specific dye. The optical density of the pink-colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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