Product Information | |
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Product Name | CD Phospholipid Assay Kit |
Applications | For quantitative determination of phospholipid and evaluation of drug effects on its metabolism. |
Features | Sensitive. Use 20 µL samples. Linear detection range: colorimetric assay 3 – 200 µM, fluorimetric assay 0.6 – 20 µM phospholipid. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. |
Detection Method | OD570nm, or FL530/585nm |
Sample Type | Biological |
Species | All |
Assay Time | 20 min |
Limit of Detection | OD, FL: 3, 0.6 µM |
Expiration Date | 6 months |
Description | Phospholipids are a class of lipids that constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. BioAssay Systems method provides a simple, direct, and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin, and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink-colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample. |
Shipping Conditions | Shipping: On Ice |
Storage | Storage: -20°C upon receipt |
Please note that all services are for research use only. Not intended for any clinical use.
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