CD Phospholipid Assay Kit

Catalog Number: AKBA351

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$1407

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Specifications

Product Information
Product Name	CD Phospholipid Assay Kit
Applications	For quantitative determination of phospholipid and evaluation of drug effects on its metabolism.
Features	Sensitive. Use 20 µL samples. Linear detection range: colorimetric assay 3 – 200 µM, fluorimetric assay 0.6 – 20 µM phospholipid. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD570nm, or FL530/585nm
Sample Type	Biological
Species	All
Assay Time	20 min
Limit of Detection	OD, FL: 3, 0.6 µM
Expiration Date	6 months
Description	Phospholipids are a class of lipids that constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. CD method provides a simple, direct, and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin, and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink-colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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