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Product Information | |
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Product Name | CD Phytase Assay Kit |
Applications | A simplest, direct “Add-and-Measure” assay for phytase enzyme activity in agricultural and biological samples. |
Features | Simple and convenient: No complex detection reagents to mix. High sensitivity and wide detection range: Detection range of 0.01 to 20 U/L phytase in a 96-well plate assay. Fast and high-throughput: Homogeneous “mix-and-measure” assay allows for quantification of phytase activity within 60 minutes. |
Detection Method | OD620nm |
Sample Type | Enzyme extracts, agriculture, and biological samples |
Species | All (e.g. Wheat, Barley) |
Assay Time | 60 min |
Limit of Detection | 0.01 U/L |
Expiration Date | 12 months |
Description | PHYTASE catalyzes the hydrolysis of phosphoester bonds on myoinositol-(1,2,3,4,5,6)-hexakisphosphate (Phytic acid or IP6) thereby releasing inositol and phosphate. Phytic acid is a major storage reservoir for phosphate in plants. Phytase is abundant in grains such as wheat and barley, and the hydrolysis of phytic acid by single-stomached animals is a crucial aspect of animal nutrition. In addition, the lack of phytase in single-stomached animals can lead to excessive phosphorus leaching into the environment due to undigested phytic acid.Simple, direct, and automation-ready procedures for measuring phytase activity are very desirable. BioAssay Systems’ QuantiChromTM Phytase Assay is based on our Malachite Green Phosphate Assay (POMG-25H). The color intensity, measured at 620 nm, is proportionate to the amount of phosphate released from phytic acid. |
Shipping Conditions | Shipping: RT |
Storage | Storage: 4°C upon receipt |
Please note that all services are for research use only. Not intended for any clinical use.
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