CD Pyrophosphatase Assay Kit

Catalog Number: AKBA175

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$1077

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Specifications

Product Information
Product Name	CD Pyrophosphatase Assay Kit
Applications	For detection and quantification of pyrophosphatase enzyme activity.
Features	Safe and sensitive. Non-radioactive assay. Use as little as 10 μL samples. Linear detection range in 96-well plate: 1.0 to 20 U/L activity. Fast and convenient. The procedure involves addition of a single working reagent and incubation for 60 min. Room temperature assay. No 37°C incubator is needed. High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day.
Detection Method	OD620
Sample Type	Biological samples
Species	All
Assay Time	1 hr
Limit of Detection	1.0 U/L
Expiration Date	6 months
Description	INORGANIC PYROPHOSPHATASE(E.C.3.6.1.1) catalyzes the hydrolysis of phosphoester bonds on inorganic pyrophosphate [P2O74-], thereby releasing two orthophosphate molecules. Family I PPases are essential enzymes found in all kingdoms of life and are responsible for maintaining the correct pyrophosphate equilibrium necessary to carry out nucleic acid and protein synthesis, and facilitate fatty acid β-oxidation. Simple, direct and automation-ready procedures for measuring pyrophosphatase activity are very desirable. BioAssay Systems’ DPPT-100 assay is based on our proprietary phosphate assay kit (POMG-25H). The color intensity, measured at 620 nm, is proportionate to the amount of phosphate released from pyrophosphate hydrolysis.
Shipping Conditions	Shipping: RT
Storage	Storage: 4°C upon receipt.

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