CD Pyrophosphatase Inhibitor Assay Kit

Catalog Number: AKBA176

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$1077

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Specifications

Product Information
Product Name	CD Pyrophosphatase Inhibitor Assay Kit
Applications	For high-throughput inhibitor screening and evaluation of pyrophosphatase modulators.
Features	Safe. Non-radioactive assay. Fast and convenient. The procedure involves incubation of enzyme and inhibitor, addition of a single working reagent and incubation for 30 min. Room temperature assay. No 37°C incubator is needed. High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems. Robust assay with a Z’ factor of 0.59. Can be used in 96-well, 384-well, and potentially higher density screening assays.
Detection Method	OD620
Sample Type	Compounds that affect pyrophosphatase activity
Species	All
Assay Time	70 min
Limit of Detection	NA
Expiration Date	6 months
Description	INORGANIC PYROPHOSPHATASE(E.C.3.6.1.1) catalyzes the hydrolysis of phosphoester bonds of inorganic pyrophosphate [P2O74-], thereby releasing two orthophosphate molecules. Family I PPases are essential enzymes found in all kingdoms of life and are responsible for maintaining the correct pyrophosphate equilibrium necessary to carry out nucleic acid and protein synthesis, and facilitate fatty acid beta oxidation.
Shipping Conditions	Shipping: RT
Storage	Storage: 4°C upon receipt.

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