CD Pyruvate Assay Kit

Catalog Number: AKBA042

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$1257

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Specifications

Product Information
Product Name	CD Pyruvate Assay Kit
Applications	For quantitative determination of pyruvate and evaluation of drug effects on its metabolism.
Features	Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 2 to 500 µM (17 µg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 µM for fluorimetric assays. Simple and convenient. The procedure involves the addition of a single working reagent and incubation for 30 min at room temperature, compatible with HTS assays. Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.
Detection Method	OD570nm, or FL530/585nm
Sample Type	Biological
Species	All
Assay Time	20 min
Limit of Detection	OD, FL: 2, 0.2 µM
Expiration Date	6 months
Description	Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine, and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct, and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery. CD pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to pyruvate concentration in the sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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