CD Pyruvate Dehydrogenase Assay Kit

Catalog Number: AKBA177

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$1167

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Specifications

Product Information
Product Name	CD Pyruvate Dehydrogenase Assay Kit
Applications	For assessing PDH activity in biological samples and screening and evaluation of PDH modulators
Features	High sensitivity and wide linear range. The detection limit is 0.87 U/L, with linearity up to at least 278 U/L PDH in a 96-well plate assay. Homogeneous and simple procedure. Simple “mix-and-measure” procedure allows reliable quantitation of PDH activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.
Detection Method	OD565nm
Sample Type	Biological samples
Species	All
Assay Time	20 min
Limit of Detection	0.87 U/L
Expiration Date	6 months
Description	PYRUVATE DEHYDROGENASE (PDH) (E.C. 1.2.4.1) is an oxidoreductase which catalyzes the decarboxylation of pyruvate to form acetylated dihydrolipoamide in a thiamine pyrophosphate (TPP)-dependent reaction. PDH is the E1 component of the PDH complex that ultimately produces acetyl-CoA. The PDH complex connects Glycolysis to the Citric Acid Cycle. This non-radioactive colorimetric PDH assay is based on the reduction of the tetrazolium salt MTT in a Phenazine Methosulfate (PMS) coupled reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the purple color formed is directly proportional to the PDH activity.
Storage	Storage: -20°C upon receipt.

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