CD Sialic Acid Assay Kit

Catalog Number: AKBA043

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$1038

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Specifications

Product Information
Product Name	CD Sialic Acid Assay Kit
Applications	For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on its metabolism.
Features	Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.02 to 1 mM sialic acid for colorimetric assays and 2 to 100 µM for fluorimetric assays. Simple and convenient. Can detect free sialic acid by the addition of a single working reagent and incubation for 60 min at room temperature or total sialic acid by pre-treating samples with a 60 min hydrolysis step.
Detection Method	OD570nm, or FL530/585nm
Sample Type	Biological
Species	All
Assay Time	2 hrs
Limit of Detection	OD, FL: 20, 2 µM
Expiration Date	6 months
Description	Sialic acid is a general name for nine-carbon acidic sugars with N- or O-substituted derivatives. The most common member of these sugars is N-acetylneuraminic acid (NANA). Sialic acid is widely distributed throughout mammalian tissues and fluids including serum. Sialylated oligosaccharides have been shown to exhibit antiviral properties and are also known to influence blood coagulation and cholesterol levels. The Sialic acid level in body fluids is also an important marker for diagnosing cancer. Simple, direct, and automation-ready procedures for measuring sialic acid concentrations find wide applications in research and drug discovery. BioAssay Systems’ sialic acid assay uses a single Working Reagent that combines NANA aldolase, pyruvate oxidase, and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to the sialic acid concentration in the sample
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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