CD Sialic Acid Assay Kit

Catalog Number: AKBA413

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$1497

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Specifications

Product Information
Product Name	CD Sialic Acid Assay Kit
Applications	For quantitative determination of free and total sialic acid (NANA) and evaluation of drug effects on sialic acid.
Features	Sensitive and accurate. Use as little as 60 µL samples. Linear detection range in 96-well plate: 5 to 1000 µM sialic acid for colorimetric assays and 0.5 to 100 µM for fluorimetric assays.
Detection Method	OD549nm, or FL555/585nm
Sample Type	Serum, plasma, saliva, milk, etc
Species	All
Assay Time	2.5 hrs
Limit of Detection	OD, FL: 5, 0.5 µM
Expiration Date	24 months
Description	SIALIC ACID is a general name for nine-carbon acidic sugars with N- or O-substituted derivatives. The most common member of these sugars is N-acetylneuraminic acid (NANA). Sialic acid is widely distributed throughout mammalian tissues and fluids including serum. Sialylated oligosaccharides have been shown to exhibit antiviral properties and are also known to influence blood coagulation and cholesterol levels. The sialic acid level in body fluids is also an important marker for diagnosing cancer. Simple and direct procedures for measuring sialic acid concentrations find wide applications in research and drug discovery. CD sialic acid assay uses an improved Warren method, in which sialic acid is oxidized to formylpyruvic acid which reacts with thiobarbituric acid to form a pink-colored product. The color intensity at 549 nm or fluorescence intensity at λex/em = 555/585 nm is directly proportional to the sialic acid concentration in the sample.
Shipping Conditions	Shipping: RT
Storage	Storage: -20°C upon receipt

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