CD Sorbitol Assay Kit

Catalog Number: AKBA145

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$1467

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Specifications

Product Information
Product Name	CD Sorbitol Assay Kit
Applications	For quantitative determination of D-sorbitol and evaluation of drug effects on sorbitol metabolism.
Features	Fast and sensitive. Linear detection range (20 µL sample): 5 to 1000 µM D-sorbitol. Convenient and high-throughput. Room temperature assay. No 37°C incubator is required. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD565nm
Sample Type	Biological (e.g. blood), food, beverage, and agriculture samples
Species	All
Assay Time	30 min
Limit of Detection	5 µM
Expiration Date	6 months
Description	SORBITOL (glucitol) is a sugar alcohol that is metabolized slowly in the human body. Sorbitol can be obtained from glucose by reducing the aldehyde group to a hydroxyl group. Accumulation of excessive sorbitol in erythrocytes, retinal cells, and Schwann cells has been associated with retinopathy, cataracts, peripheral neuropathy, and diabetes. Sorbitol is made solely from corn syrup, and found in fruits such as apples, pears, peaches, and prunes. It is widely used as a sugar substitute and as a laxative. It is also utilized in specialty culture media and in healthcare, food, and cosmetic products. Sorbitol is measured in biological samples to monitor metabolic pathways and the progression of diabetes. CD sorbitol assay involves an end-point enzyme coupled MTT/NAD reaction that forms a colored product with an absorption maximum of 565 nm. The increase in absorbance at 565 nm is directly proportional to the sorbitol concentration.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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