CD Sorbitol Dehydrogenase Assay Kit

Catalog Number: AKBA178

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$1407

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Specifications

Product Information
Product Name	CD Sorbitol Dehydrogenase Assay Kit
Applications	For quantitative determination of sorbitol dehydrogenase enzyme activity.
Features	Fast and sensitive. Linear detection range (20 µL sample): 0.1 to 125 U/L for 12 min reaction. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD565nm
Sample Type	Biological samples e.g. plasma, serum, urine, tissue, and culture media
Species	All
Assay Time	15 min
Limit of Detection	0.1 U/L
Expiration Date	6 months
Description	SORBITOL DEHYDROGENASE (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage; thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy. BioAssay Systems’ non-radioactive, colorimetric SDH assay is based on the reduction of the tetrazolium salt MTT in an NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
Shipping Conditions	Shipping: RT
Storage	Storage: -20°C upon receipt

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