CD Sphingomyelin Assay Kit

Catalog Number: AKBA146

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$1437

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Specifications

Product Information
Product Name	CD Sphingomyelin Assay Kit
Applications	For quantitative determination of sphingomyelin in biological samples (serum, plasma, etc.).
Features	Safe. Non-radioactive assay. Sensitive and accurate. Linear detection range in a 96-well plate: 1 – 50 mg/dL sphingomyelin for colorimetric assays and 0.07 – 7 mg/dL sphingomyelin for fluorimetric assays. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day.
Detection Method	OD570nm or FL530/585nm
Sample Type	Serum, plasma, and other biological samples
Species	All
Assay Time	30 min
Limit of Detection	OD, FL: 1, 0.07 mg/dL
Expiration Date	6 months
Description	SPHINGOMYELINS (ceramide phosphocholine, SM) are key lipid components of cell membranes and lipoproteins. Sphingomyelinases (SMases) hydrolyze most sphingomyelins into ceramide and phosphocholine. This release of ceramide contributes to cell signaling pathways resulting in cell proliferation, differentiation, growth arrest, and apoptosis. Niemann-Pick disease is identified by the accumulation of sphingomyelin due to deficiencies in sphingomyelinase activity. Increased sphingomyelin levels have also been observed in several diseases, such as atherosclerosis, so simple, direct, and automation-ready procedures for measuring sphingomyelins are very desirable. BioAssay Systems’ Sphingomyelin Assay Kit provides a convenient colorimetric and fluorimetric method to quantify sphingomyelin in biological samples. In this assay, sphingomyelin is enzymatically converted to hydrogen peroxide, which forms a colored product with the dye reagent that is measurable at OD570 nm or fluorescence at λex/em = 530/585nm. The colored product is directly proportional to the sphingomyelin concentration in the sample.
Storage	Storage: -20°C upon receipt.

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