CD Superoxide Dismutase Assay Kit

Catalog Number: AKBA208

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$1167

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Specifications

Product Information
Product Name	CD Superoxide Dismutase Assay Kit
Applications	For quantitative determination of superoxide dismutase (SOD) enzyme activity and evaluation of drug effects.
Features	Sensitive and accurate. Linear detection range of 0.05 – 3 U/mL SOD. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD440nm
Sample Type	Blood, cell, tissue, etc
Species	All
Assay Time	1 hr
Limit of Detection	0.05 U/mL
Expiration Date	6 months
Description	Superoxide dismutases (SOD, EC1.15.1.1) are enzymes that catalyze the dismutation of superoxide into O2 and H2O2. They are an important antioxidant defense in all cells exposed to O2. There are three major families of superoxide dismutase: Cu/Zn, Fe/Mn, and the Ni type. Aberrant SOD activities have been linked to diseases such as amyotrophic lateral sclerosis, perinatal lethality, neural disorders, and cancer. CD SOD assay provides a convenient colorimetric means for the quantitative determination of SOD enzyme activity in biological samples. In the assay, superoxide (O2–) is provided by a xanthine oxidase (XO) catalyzed reaction. O2– reacts with a WST-1 dye to form a colored product. SOD scavenges the O2– thus less O2– is available for the chromogenic reaction. The color intensity (OD440nm) is used to determine the SOD activity in a sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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