Cell-Based Protein Expression

CD Biosynsis has a team of experienced and highly skilled scientists to provide synthetic biology researchers around the world with high-quality cell-based protein expression services to facilitate their cutting-edge research.

Introduction to Cell-Based Protein Expression

Methods of producing or manufacturing functional proteins are necessary to study the biological roles of proteins of interest. Protein expression can be used across diverse areas of life science research. It has been the core activity of high throughput screening, functional validation, and structural biology. Many synthetic biology methods also rely on protein expression.

Given the different sizes, characterization, and complexity of proteins, different cell-based expression systems, including both prokaryotic and eukaryotic protein expression systems, have been developed. Each system has its own advantages and challenges. For protein expression to be successful, selecting a suitable system for the specific application is essential. The selection is mainly based on protein type, protein yield, and the requirements for functional activity.

Figure 1. Comparison of various commercial recombinant protein expression systems. (Raskin I, et al., 2002)

Service Offering

CD Biosynsis offers various cell-based protein expression systems and related services as follows to give you the maximum flexibility to meet your needs.

Expression System	Main Applications	Advantages
Bacteria Expression System	Antibody production Structural analysis Functional analysis	Lower production costs High level of expression Simple culture conditions and rapid biomass accumulation High-throughput capabilities Simple process scale-up Easy for purification Wide application range
Mammalian Expression System	Antibody production Structural analysis Functional analysis Complex protein expression Virus production	High expression level Well-characterized genetic background More efficient and proper protein folding and post-translational modification. Easy for purification. Suitable for large proteins. High-throughput capabilities.
Yeast Expression System	Antibody production Structural analysis Functional analysis	Easy to manipulate and fermentation High level of expression Simple culture conditions and cost-effective Possible protein folding and post-translational modification Without endotoxin
Insect Expression System	Antibody production Structural analysis Functional analysis Complex protein expression Intracellular protein expression Virus production	Moderately rapid expression High expression level Detergent-free Possible protein folding and post-translational modification Suitable for large proteins and protein complexes
Algal Expression System	Photosynthesis study Plant biology study Lipid metabolism study Genetic engineering Biofuel production	Excellent experimental control for biofuel and specialty chemical production Fast growth rates Covering all advantages of eukaryotic expression systems

Why CD Biosynsis?

CD Biosynsis provides a variety of cutting-edge techniques for cell-based protein expression. Since there is no standard approach for the expression of each different protein, our scientists can use their experience and expertise in protein expression to provide the best tailor-made services for specific needs.

If you are interested in our services or have any specific needs, please feel free to contact us for more details.

References

1. Raskin I, et al. Plants and human health in the twenty-first century. TRENDS in Biotechnology, 2002, 20(12): 522-531.
2. Tate C G, et al. Comparison of seven different heterologous protein expression systems for the production of the serotonin transporter. Biochimica et Biophysica Acta (BBA)-Biomembranes, 2003, 1610(1): 141-153.

Please note that all services are for research use only. Not intended for any clinical use.