Cell-Free Antibody Production Service

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We offer a state-of-the-art Cell-Free Antibody Production Service designed to streamline and accelerate the production of high-quality antibodies. Our advanced cell-free technology bypasses the limitations of traditional cell-based systems, offering rapid and scalable production of antibodies. Whether you are in pharmaceutical research, diagnostics, or therapeutic development, our cell-free antibody production service can meet your specific needs with unmatched efficiency and precision.

Scheme showing the cell-free synthesis of antibodies and antibody fragments in prokaryotic in vitro translation systems(M Stech, et al.,2015)

Overview Service Process Examples and Solutions Frequently Asked Questions

Overview

The generation of antibodies by hybridoma technology, as well as the synthesis of antibodies and antibody fragments in prokaryotic and mammalian cells are well-established. However, a cell-based production of target proteins is known to be rather time-consuming and cost-intensive, thus resulting in the development of efficient and powerful cell-free protein synthesis systems. Over the last decade, cellfree systems have proven themselves as reliable, flexible and potent protein production platform, providing proteins of varying origins, classes and properties [3–7]. In particular, cell-free systems have been successfully used for the synthesis of antibodies and antibody fragments. Since this class of molecules is highly in demand.

Cell-free antibody production leverages a cell-free protein synthesis system that uses extracts from cells to synthesize proteins in vitro. This approach eliminates the need for living cells, reducing the time and complexity associated with traditional antibody production methods. Below is a comparison of cell-free antibody production and traditional cell-based methods:

Method	Description	Advantages	Applications
Cell-Free System	In vitro protein synthesis using cell extracts.	Rapid production, scalable, flexible, no need for cell culture.	High-throughput screening, therapeutic antibody development, diagnostic assays.
Cell-Based System	Antibody production using living cells (e.g., CHO, HEK293).	High yield, post-translational modifications.	Therapeutic antibody production, research applications.

Service Process

Project Consultation: Collaborating with researchers to define specific antibody production goals, including target antigens, desired modifications, and intended applications.
Gene Synthesis and Cloning: Synthesizing and cloning the gene of interest into suitable vectors optimized for cell-free expression.
Expression Optimization: Optimizing cell-free reaction conditions to achieve high yield and functionality of antibodies.
Antibody Purification: Purifying produced antibodies using advanced chromatography techniques to ensure high purity.
Quality Assurance: Performing stringent quality control assays to validate antibody purity and functionality.
Delivery and Support: Delivering purified antibodies with comprehensive documentation and providing ongoing technical support.

For more information on our cell-free antibody production service, please fill out our online inquiry form. One of our specialists will get back to you promptly to discuss your specific needs and how we can assist you in achieving your research goals. We are committed to providing personalized support and ensuring the success of your projects with our high-quality antibody production services.

Examples and Solutions

Case Examples	Solutions We Offer
High-Throughput Screening: Rapid production of a large panel of antibodies for screening potential drug targets.	Utilizing our cell-free system to quickly produce and screen multiple antibodies, saving time and resources.
Therapeutic Development: Development of therapeutic antibodies against novel targets.	Providing scalable and efficient antibody production to support preclinical and clinical development stages.
Diagnostic Assays: Production of specific antibodies for diagnostic kit development.	Ensuring high specificity and sensitivity of antibodies produced, tailored for diagnostic applications.
Research Applications: Custom antibody production for various research needs.	Offering flexible production options to meet unique research requirements, including modifications and labeling.
Biophysical Studies: Antibody production for structural and interaction studies.	Producing antibodies optimized for biophysical and structural analysis, ensuring functional integrity.
Enzyme-Linked Immunosorbent Assay (ELISA): Production of antibodies for use in ELISA kits.	Delivering high-affinity antibodies suitable for quantitative and qualitative assays.

Frequently Asked Questions

Q: What is cell-free antibody production?

A: Cell-free antibody production involves synthesizing antibodies in vitro using cell extracts, eliminating the need for living cells and enabling rapid and scalable production.

Q: What are the benefits of using a cell-free system for antibody production?

A: Cell-free systems offer rapid production, scalability, flexibility, and do not require cell culture, making them ideal for high-throughput applications and fast turnaround times.

Q: Can you produce modified antibodies using the cell-free system?

A: Yes, we can produce antibodies with various modifications, including fragment formats, fusion proteins, and labeled antibodies, tailored to your specific needs.

Q: How do you ensure the quality of antibodies produced?

A: We employ stringent purification and characterization techniques to ensure high purity and functionality, including quality control assays to validate antibody integrity.

Q: What documentation do you provide with the delivered antibodies?

A: We provide detailed reports on expression conditions, purification methods, and quality control assays, ensuring transparency and reproducibility.

Q: What kind of support do you offer post-delivery?

A: We offer ongoing technical support and troubleshooting assistance to ensure the successful utilization of the delivered antibodies in your applications.

Please note that all services are for research use only. Not intended for any clinical use.

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