We provide an innovative Cell-Free Membrane Protein Expression Service designed to overcome the challenges associated with membrane protein production. Our advanced cell-free technology allows for the rapid and efficient synthesis of membrane proteins without the need for living cells. This approach is particularly beneficial for producing complex and difficult-to-express membrane proteins, which are essential for a wide range of applications in drug discovery, structural biology, and biotechnology.
Different modes for CF expression of MPs(C Klammt, et al.,2006)
Membrane proteins are critical to numerous biological processes and are key targets in drug discovery and development. However, their hydrophobic nature and complex folding requirements make them challenging to express using traditional cell-based systems. Our cell-free expression platform overcomes these challenges, offering several advantages:
For more information on our cell-free membrane protein expression service, please fill out our online inquiry form. One of our specialists will get back to you promptly to discuss your specific needs and how we can assist you in achieving your goals.
Case Examples | Solutions We Offer |
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Drug Discovery: Rapid production of membrane proteins for screening potential drug candidates. | Utilizing our cell-free system to quickly produce high-quality membrane proteins, facilitating faster drug discovery processes. |
Structural Biology: Production of membrane proteins for structural analysis. | Providing scalable production of membrane proteins suitable for crystallization and NMR studies. |
Functional Assays: Need for functional membrane proteins for various assays. | Delivering active membrane proteins that retain functionality, suitable for a variety of biochemical and biophysical assays. |
High-Throughput Screening: Requirement of membrane proteins for high-throughput screening assays. | Offering scalable and efficient production to meet high-demand screening needs. |
Protein-Protein Interaction Studies: Investigating interactions between membrane proteins and other biomolecules. | Producing high-quality membrane proteins for detailed interaction studies and binding assays. |
Enzyme Activity Studies: Analyzing the activity of membrane-bound enzymes. | Ensuring functional integrity of membrane proteins for accurate enzymatic assays. |
Vaccine Development: Production of membrane proteins as vaccine targets. | Providing high-purity membrane proteins for use in vaccine research and development. |
A: Cell-free membrane protein expression involves synthesizing membrane proteins in vitro using cell extracts, eliminating the need for living cells and enabling rapid and efficient production.
A: Cell-free systems offer rapid production, flexibility, and are particularly suitable for difficult-to-express proteins. They also eliminate the complexities of cell culture, making them ideal for high-throughput applications.
A: Yes, we can produce membrane proteins with various modifications, including tagged versions, fusion proteins, and other customized formats tailored to your specific needs.
A: We employ stringent purification and characterization techniques to ensure high purity and functionality, including quality control assays to validate protein integrity.
A: We provide detailed reports on expression conditions, purification methods, and quality control assays, ensuring transparency and reproducibility.
A: We offer ongoing technical support and troubleshooting assistance to ensure the successful utilization of the delivered membrane proteins in your applications.
Please note that all services are for research use only. Not intended for any clinical use.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
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CD Biosynsis is a leading customer-focused biotechnology company dedicated to providing high-quality products, comprehensive service packages, and tailored solutions to support and facilitate the applications of synthetic biology in a wide range of areas.