Cell-Free Systems

Introduction of Cell-Free Systems

Cell-free systems are gaining popularity as a platform for protein synthesis due to their rapid and efficient nature. These systems are free from cell walls and membranes and can be used to synthesize proteins directly from DNA templates. They offer several advantages over traditional methods of protein expression, including increased speed, simplicity, and cost-effectiveness. Synthetic biology has played a significant role in advancing cell-free systems by providing new methods for DNA synthesis and assembly, as well as new enzymes and substrates for protein synthesis.

CD Biosynsis is a leading company in the field of synthetic biology and protein expression. With years of experience in the industry, our team of experts has developed a range of services for cell-free systems that can help researchers to achieve their goals in a more efficient and cost-effective way.

Figure 1: Key advantages of cellular and cell-free bioproduction. (Claassens Nico J., et al. 2019)

Our Services for Cell-Free Systems

At CD Biosynsis, we offer a comprehensive range of services for cell-free systems that can be tailored to meet the specific needs of our clients. Our services include DNA synthesis, DNA assembly, protein expression, and purification.

DNA Synthesis

• Custom DNA synthesis up to 10 kb in length
• High fidelity and purity of synthesized DNA fragments
• Codon optimization to enhance protein expression
• Mutagenesis to introduce specific mutations or modifications
• Cloning of DNA fragments into vectors for protein expression.

DNA Assembly

• Gibson assembly to construct complex DNA sequences from smaller fragments
• Golden Gate assembly to assemble multiple DNA fragments in a single reaction
• Advanced methods to ensure high efficiency and accuracy in the assembly process
• Customized assembly strategies to meet the specific needs of our clients.

Protein Expression

• High-yield protein expression using our state-of-the-art E. coli cell-free system
• Rapid and efficient protein expression in a matter of hours
• Both batch and continuous-flow systems available
• Minimal equipment and reagents required
• Customized solutions to meet the specific needs of our clients
• Guidance and support from our team of experts throughout the entire protein expression process.

Protein Purification

• Isolation and purification of the synthesized protein
• Affinity chromatography, ion exchange chromatography, size exclusion chromatography, and other advanced methods available
• Customized purification strategies to meet the specific needs of our clients
• High purity and yield of the synthesized protein

Customized Solutions

Our Advantages

• Experience and Expertise
• Customization and Flexibility
• High Quality
• Cost-effectiveness

At CD Biosynsis, we offer a comprehensive range of services for cell-free systems, including DNA synthesis, DNA assembly, protein expression, and protein purification. Our team of experts has extensive experience in synthetic biology and protein expression and can provide customized solutions to meet the specific needs of our clients. We are committed to quality and customer satisfaction and strive to provide the highest value to our clients. Contact us to find more our services.

References

1. Claassens N. J., et al. A critical comparison of cellular and cell-free bioproduction systems. Current Opinion in Biotechnology, 2019, 60: 221-229.
2. Karig D. K., et al. Expression optimization and synthetic gene networks in cell-free systems. Nucleic Acids Research, 2012, 40(8): 3763-3774.

Please note that all services are for research use only. Not intended for any clinical use.